Fluorescence Measurements
We will assist you in selection and purchase of excitation and emission filters
tailored to your application. Contact us at fialab@flowinjection.com
Voice 425-376-0450 or 1-800-963-1101, Fax 425-376-0451
We offer both advanced fiber optic based flow cells, as well as conventional instruments where a flow through cuvette is interfaced directly with a PMT and illuminating light source. While fiber optic
fluorometers are more versatile and more compatible with microfluidic systems like micro SI, the traditional systems offer the ultimate when the lowest possible detection limit of fluorescence emission is desired.
Fiber Optic Flowcell
The SMA-FL Fluorescence Flow Cell utilizes a fiber optic cable to send excitation energy through a fused silica window into a 2-mm inner-diameter sample compartment. Emitted energy is collected by a second fiber, oriented at 90°, that connects to a spectrometer/detector configured for fluorescence. The flow cell is available in chemically inert Black Ultem. The cell is compatible with standard SMA 905 terminated fiber optics. The windows are removable for cleaning or replacement to another material with different optical properties.
Each cell includes 1 mm thick UV grade Fused Silica windows, a hard, non-corrosive, chemically resistant material that also has a high UV through IR transmission (260 nm through 2000 nm). The windows are held in place by chemically resistant seals, pressure mounted with steel SMA connectors (connectors do not contact the fluids).
Each order includes one SMA-FL-Cell with fused silica windows, two steel SMA connectors, six chemically resistant Upchurch tubing connectors, two meters of Teflon tubing, six chemically resistant seals. Two SMA terminated fiber optic cables P600-20 are required and must be purchased separately.
Recommended Instrument Configurations using Fiber Optics
The Versatile Fluorometer
The system comprises OO Spectrofluorometer and Tungsten or Deuterium light excitation source, a pair of quartz optical fibers and an SMA-FL-CELL. For exploratory work, no excitation or emission filters are required.
High Sensitivity Fluorescence Measurement
PMT Specifications: Photon counting PMT detector 310nm - 750nm spectral range. Pulse pair resolution: 10ns responsivity @ 400nm: 5 X 1017 cps/Watt PMT dynamic range: 2.0E06 The PMT-FL is built to order with either an internal excitation lamp or an SMA-terminated fiber optic cable for use with an external lamp. Emission and excitation filters are mounted in user accessible slots inside the enclosure, allowing for quick removal for exchange.
Additional Components
Built in bandpass excitation and/or emission filters. We offer numerous bandpass filters for fluorometric assays, from the UV through VIS. Please inquire for technical and pricing specifics.
Coupled LED based excitation source. LEDs from 270 nm to 920 nm are available. These can be directly coupled to the flowcell. Please inquire for technical and pricing specifics.
Conventional Configuration for Ultra High Sensitivity Fluorescence Measurement
The PMT-FL Flow Through Fluorometer is an ultra sensitive photomultiplier- based flow through detection system designed for monitoring of very low fluorescence, chemiluminescence or bioluminescence emissions.
The heavy-duty light tight chemically resistant housing is built to withstand industrial environments.
The PMT- FL accepts either a flow through or a conventional quartz cuvette for manual sample handling. The detection limit is guaranteed to be 10 parts per trillion - obtained with Fluorescein @ 250 msec integration time and appropriate emission and excitation filters.
Compatible with FIAlab 2500, microSI or FIAlab 3200 or with a mini perstaltic pump for manual operation.
For information on excitation sources consult the page Light Sources.
The PMT-FL with the MicroSIA system
PMT-FL shown with an S2-Mini Peristaltic Pump
